FastQCFastQC Report
Sun 14 Apr 2019
SRR6576518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264868
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7470.28202727396287963No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4750.17933461195765438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.1683857619644502No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3870.1461105154265521No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3800.1434676895661235No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3440.12987601371249075No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3160.11930471027077638No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3090.1166618844103478No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3060.11552924475587839No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2860.10797831372608242No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2780.10495794131416404No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2680.10118247579926605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7800.042.7955471
CGCTAAA450.001013793341.767295
GTATAAC500.001697030737.6118621
CCGCTAA500.00170176137.590564
GTACATG17250.036.5216641
ACATGGG17700.035.307523
CATGGGT5000.034.7712674
TACATGG18150.034.7106742
AACGCAG9650.034.571636
CCGAACT550.002709056334.1926041
ATAATAC702.1837001E-433.5633
ATCAACG10000.033.361623
TCAACGC10150.032.8685954
CAACGCA10300.032.3899235
GGTACCT2950.031.856418
TGCCCGC1052.0523803E-631.343221
ATGGGTA3300.031.3254665
CATGGGG9550.030.9974524
ACGCAGA11350.029.3935017
CGCAGAG11450.029.136798
Produced by FastQC (version 0.11.2)