FastQCFastQC Report
Sun 14 Apr 2019
SRR6576518_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576518_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264868
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7200.2718335170726551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.2031200447015117No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4860.18348762402404215No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4020.15177371369889908No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3890.14686560852953168No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3850.1453554223235725No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3580.13516166543334793No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3560.13440657233036835No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3310.12496790854312338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3110.11741697751332739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.11515169820438861No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3030.114396605101409No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3010.1136415119984294No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2870.10835586027757223No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2820.10646812752012323No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2700.10193756890224563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6400.047.82411
GGTATCA3150.043.3511771
TAATAGT450.001013542441.769394
TTAGACG450.001013542441.769394
GTACATG12350.040.0346261
ACATGGG13000.037.592453
TACATGG13250.037.2378042
AACGCAG9150.034.408396
TAGTACT550.002715925234.1749534
TCAACGC9250.033.0203974
CAACGCA9350.032.6672335
ATCAACG9350.032.6672333
CATGGGT4200.032.445864
AGACGTC600.004159341631.327046
CATGGGG7100.031.1064284
TGGGACG1154.186926E-628.602955
TATCAAC11450.028.3174552
CATGGGA3000.028.1943384
ACGCAGA11500.027.377117
GTATGGA700.008743498526.9076251
Produced by FastQC (version 0.11.2)