FastQCFastQC Report
Sun 14 Apr 2019
SRR6576519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606302
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10650.17565503659892265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.14514218986577646No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7150.11792802926594338No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6560.10819690517266972No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6370.10506315334602229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.052.877651
ATCAACG16000.043.183
TCAACGC16900.040.8804744
CAACGCA17350.040.3619465
AACGCAG18350.038.930756
GTACATG32350.038.8735731
TACATGG33600.037.2072562
ACATGGG33900.037.016633
CATGGGG14200.034.4215354
TATCAAC20350.034.1808322
CGCAGAG21000.033.794268
ACGCAGA21650.032.9967357
CATGGGT9850.030.5371864
GGTATCA5150.028.351231
GCAGAGT25600.027.9054439
ATGGGTA5800.027.5509265
ATGGGGA8400.027.4158735
CATGGGA14100.026.6658944
ATGGGGG7250.026.578545
AGAGTAC24050.023.05962410-11
Produced by FastQC (version 0.11.2)