FastQCFastQC Report
Sun 14 Apr 2019
SRR6576520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556547
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10960.1969285612895227No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7560.13583758424715253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.13350175277200307No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6460.11607285638050334No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6050.10870600326657047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11000.057.6812671
ATCAACG14900.041.6259653
CAACGCA14950.041.4867445
TCAACGC15050.041.2110824
AACGCAG15250.040.6706126
GTACATG28950.040.424481
TACATGG30400.038.1871342
ACATGGG29850.038.093223
GGTATCA4150.035.1080971
TATCAAC18200.034.6040652
ACGCAGA18950.032.97767
CGCAGAG18850.032.9032828
GGTACCT4300.032.7815488
ATGGGTA4800.032.3034865
CATGGGT9650.032.136114
CATGGGG12300.032.0886044
CTCGATT600.00416300831.32740294
CATGGGA12450.030.9471844
ATACCGT953.7559847E-529.6759246
GGGTACC4300.029.5033917
Produced by FastQC (version 0.11.2)