FastQCFastQC Report
Sun 14 Apr 2019
SRR6576521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166395
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG27430.23516904650654366No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA16330.14000402950972868No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13780.11814179587532525No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT13730.11771312462759186No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA13570.11634137663484496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12810.10982557366929727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21100.059.7107121
ATCAACG28850.042.5189823
GGTATCA8000.042.3099371
TCAACGC29250.042.09824
CAACGCA29400.042.0432785
AACGCAG30650.040.635316
TATCAAC35450.035.2748572
GTACATG58050.034.4180761
TACATGG58750.034.0079882
ACATGGG57650.033.995773
ACGCAGA37250.033.4354937
CGCAGAG39000.031.8146748
CATGGGG33950.029.2099514
ATGGGTA12000.028.9826855
CATGGGT16950.028.8371144
GTATATA3350.028.0662941
GCAGAGT46750.026.6411159
TGGGTAC12700.024.054586
GGTACCT12550.023.9675928
GGGTACC13050.023.409447