FastQCFastQC Report
Sun 14 Apr 2019
SRR6576521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166395
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG24970.21407842111806036No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA15610.13383116354236774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.11882766987169868No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA13600.11659857938348502No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13460.1153982998898315No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12730.10913969967292383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22400.058.0267071
ATCAACG31100.040.200443
TCAACGC30900.040.156424
CAACGCA31000.039.4204145
AACGCAG32350.037.630076
GGTATCA8900.035.982041
ACATGGG54000.035.076873
GTACATG56400.034.8196031
TACATGG55800.034.703442
TATCAAC38200.033.220792
ACGCAGA38100.031.8248987
ACCGTAT1052.0663483E-631.331498
CGCAGAG39500.030.8159088
GTATAAG3600.030.0879211
ATGGGTA11600.029.9835685
TACCGTA1102.969855E-629.9073337
CATGGGT16200.029.0131264
CATGGGG31600.028.7064694
GGTACCT11700.026.5112618
TAAACCG909.516269E-426.1118135
Produced by FastQC (version 0.11.2)