FastQCFastQC Report
Sun 14 Apr 2019
SRR6576522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048516
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG26470.2524520369741616No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA16030.15288274094052928No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA14050.13399890893415076No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12910.12312639959714491No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12830.12236341648577609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.11883461959569526No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10540.10052302492284335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21000.058.8591581
CAACGCA27800.043.9443055
TCAACGC28050.043.5526434
ATCAACG28200.043.320983
AACGCAG29100.042.465556
GTACATG49050.035.931041
ACGCAGA34550.035.902937
ACATGGG48700.035.794723
TACATGG50100.035.176322
CATGGGT14300.035.1578064
TATCAAC35000.034.776722
CGCAGAG36250.034.3488248
GGTATCA7950.033.696541
ATGGGTA10650.033.0891535
GGTACCT10000.031.4810228
GCAGAGT42900.029.0243559
CATGGGG29250.027.7903654
GTATTAC1904.474714E-1027.2092551
GGGTACC11900.026.454647
TGGGTAC12700.024.7882066
Produced by FastQC (version 0.11.2)