FastQCFastQC Report
Sun 14 Apr 2019
SRR6576522_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576522_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048516
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG25050.23890908674736483No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA14340.13676472271286275No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13150.12541534893125142No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13140.1253199760423303No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA13080.12474773870880368No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT12250.11683178892835207No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG11490.1095834493703482No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG10540.10052302492284335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19550.051.525511
ACATGGG49500.038.7314763
GTACATG50900.038.5632441
TCAACGC25600.038.363254
TACATGG51050.038.107782
ATCAACG26100.037.6283233
CAACGCA26500.037.062115
GGTATCA8200.036.164461
AACGCAG27700.035.6261836
CATGGGT14950.034.260554
CATGGGG29900.032.6889654
ACGCAGA32350.030.5052627
ATGGGTA11000.030.331615
TATCAAC32750.030.2747252
CGCAGAG33850.029.3118768
GGGTACC10500.029.0906757
GGTACCT10600.028.3918658
TGGGTAC11650.026.2190676
ATGGGGG12900.024.771315
ATGGGGA16150.024.4419885