FastQCFastQC Report
Sun 14 Apr 2019
SRR6576523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502450
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG18250.3632202209175042No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11180.22250970245795604No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA10180.20260722459946265No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9650.19205891133446112No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT8620.17155935914021295No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA8080.16081202109662654No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7980.15882177331077718No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7740.15404517862473877No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG7630.1518559060603045No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7240.1440939396954921No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC7150.14230271668822767No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT7140.14210369190964275No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT6250.12439048661558363No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG6230.12399243705841377No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC6010.11961389192954523No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5670.11284704945765747No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC5590.11125485122897802No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5560.1106577768932232No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT5530.1100607025574684No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5420.10787142999303413No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG5200.1034928848641656No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG5190.10329386008558064No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG5170.10289581052841078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT354.635913E-640.28413466-67
GTATCAA9500.040.1921041
TACATGG26850.036.758342
GTACATG27250.036.5002021
CTAGTAC801.1576638E-535.248623
ACATGGG27450.034.9275933
ACCTAAG2000.032.997221
GGTACCT6200.032.59558
CATGGGT8400.031.8916074
ATGGGTA6500.031.8141385
TGGGTAC6800.031.1017236
CTAAGAC2000.030.5488033
CATGGGG14700.029.4138154
GGGTACC7150.027.607317
CCTAGTA1205.836382E-627.4155942
TCAACGC13500.027.1544914
GCTATAT700.00870518526.9365061
GCCTAGT1406.1945684E-726.9365061
TATACTC700.00883306326.856095
AACGCAG13850.026.8076136
Produced by FastQC (version 0.11.2)