FastQCFastQC Report
Sun 14 Apr 2019
SRR6576525_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576525_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370001
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.1705400796214064No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3920.10594565960632539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7400.050.9761621
ATCAACG9850.037.690343
GGTATCA3200.036.838241
TCAACGC10150.036.5763364
CAACGCA10050.036.4726835
GTACATG16300.033.55671
AACGCAG11000.032.8955546
ACATGGG15650.032.73043
TACATGG16450.032.5758322
TATCAAC11600.032.0129472
TCGGTTG600.00415736931.33333494
TACGGAC404.7792555E-429.37570-71
GTATACG404.7792555E-429.37566-67
GGTATAT650.006051996729.01721
GTCGTGC650.006152910628.9191678
ACGCAGA12800.028.6367557
AGTCGTG1005.318813E-528.196197
CATGGGA9350.027.6433244
GTGATCG856.812673E-427.6433228
CGCAGAG13350.027.4569638
Produced by FastQC (version 0.11.2)