FastQCFastQC Report
Sun 14 Apr 2019
SRR6576526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264767
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8910.3365223007398958No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6050.2285027967986947No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4490.16958306737622136No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4440.16769461451011644No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4440.16769461451011644No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4280.16165156533858072No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3790.14314472725075253No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3720.14050089323820567No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3570.13483553463989092No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3520.132947081773786No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3290.1242601985897034No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3150.11897253056460963No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3080.11632869655206275No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3050.11519562483239981No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3010.11368486253951589No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2950.11141871910018998No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2890.10915257566086409No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.10499797935543326No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC250.00516669956.5781361
TATTACC352.9342773E-453.7109382
GTATCAA7200.042.5645681
TCAACGC7800.039.1642274
ATCAACG8100.037.71373
TATGGGT500.001700182837.597664
AACGCAG8250.037.0279966
CAACGCA8300.036.8049355
ATGGGGG2850.036.2784425
TACATGG14800.035.565352
ACATGGG14950.035.5228733
GTACATG14700.035.281151
ATCGTAG550.002671607334.289781
CATGGGG7200.033.942334
TGTAGGG702.1812788E-433.5693362
CACGACG300.00416024231.3313832-33
CGCAGAG10000.030.5480988
ACGCAGA10250.029.8030227
TATCAAC11000.029.479982
TATAGGA804.7728047E-429.373172
Produced by FastQC (version 0.11.2)