FastQCFastQC Report
Sun 14 Apr 2019
SRR6576529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317594
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6250.19679213083370592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.13696732306025933No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3640.11461173699755033No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3570.11240766513221283No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3340.10516571471753246No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3180.10012783616818957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9650.038.0248031
GTACATG20100.035.1070941
ACATGGG19450.034.7907373
TACATGG20200.033.9642642
AACGCAG11200.032.3067636
GTATCAC753.2525446E-431.3623371
CATGGGG7650.031.3277724
ATCAACG11700.031.327773
TATTAGT1052.0620027E-631.327772
CAACGCA12000.030.5445775
TCAACGC12000.030.5445774
GGGTACC2850.028.030117
CATGGGT5250.027.7474524
ACGCAGA13100.027.621057
CGCAGAG13200.027.4333958
GGTACCT3100.027.3069748
TATCAAC13850.026.4646132
TCTATAC1258.011069E-626.3153273
ATGGGCG909.5123163E-426.1064765
ATGGGTA2900.025.926435
Produced by FastQC (version 0.11.2)