FastQCFastQC Report
Sun 14 Apr 2019
SRR6576530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261001
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4460.17088057133880713No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3130.11992291217275029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5550.049.126331
ATCAACG7800.034.9485743
AACGCAG8100.034.814676
CAACGCA8000.034.6623575
GTACATG16250.034.4249151
TCAACGC8000.034.074864
TACATGG16150.033.473772
ACATGGG16600.033.126373
ATACGCT1658.54925E-1131.3332068
CATGGGA9150.029.7922294
TATCAAC9200.029.6359922
TACGCTA1751.70985E-1029.5427389
GCCCTAC804.7656437E-429.3805081
CTTATAG804.7709947E-429.374883
TATTAAC650.006139946628.9285012
TATACGC1802.3646862E-1028.7221077
ATATACG1853.255991E-1027.9458336
ATGGGAG4150.027.1806135
ACGCAGA10400.027.1152747
CGGAATT350.00883185926.85703348-49
Produced by FastQC (version 0.11.2)