FastQCFastQC Report
Sun 14 Apr 2019
SRR6576530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261001
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5010.19195328753529678No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3080.11800721070034215No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.116091509227934No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3000.1149420883444891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5900.062.2617341
ATCAACG7900.047.5910533
AACGCAG8500.045.337486
CAACGCA8350.045.0262685
TCAACGC8400.044.7582554
TATCAAC9550.040.3527342
GTACATG14450.039.4362951
GGTATCA1800.039.2461781
ACATGGG14900.038.4800833
TACATGG14950.038.3513872
ACGCAGA10100.037.224697
CGCAGAG10450.035.9779328
CATGGGG5600.034.407914
GTAATAG702.154569E-433.639581
GCAGAGT11350.033.1250539
TCCCTAC600.00415680431.3307768
ATGGGGA3200.030.8412385
GTAGAAC804.773158E-429.3726063
ATAGTGC650.006148023528.920728
CATGGGA9000.028.7198814
Produced by FastQC (version 0.11.2)