FastQCFastQC Report
Sun 14 Apr 2019
SRR6576532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299957
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12230.4077251072653747No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9680.32271292218551323No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7890.26303770207063015No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7250.2417013105211747No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6240.20802981760719036No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6070.20236233860186628No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6060.20202895748390604No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6000.20002867077614458No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5780.19269428618101928No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5700.19002723723733736No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5570.1856932827038542No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5190.17302480022136507No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4910.1636901289184783No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG4890.16302336668255782No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4810.1603563177388759No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4640.1546888387335518No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4480.14935474084618797No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4480.14935474084618797No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC4470.1490213597282277No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG4380.14602092966658553No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4260.14202035625106266No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG3980.1326856849481759No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3970.13235230383021568No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG3750.12501791923509037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.12268425140936869No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT3680.12268425140936869No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3660.1220174891734482No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG3640.1213507269375277No Hit
GCATTTATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCC3580.11935044022976626No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCT3540.1180169157579253No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG3480.11601662905016386No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC3450.11501648569628314No Hit
CTCCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG3410.11368296122444216No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG3390.1130161989885217No Hit
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG3380.11268281787056145No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT3200.10668195774727711No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC3130.10434828992155543No Hit
CATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGA3100.10334814656767471No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA3050.1016812409778735No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA3020.10068109762399277No Hit
GGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAAC3000.10001433538807229No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGT405.670567E-446.9993
ATGGGTA3850.043.9471175
GTATCAA8600.042.7124371
GGGTACC3550.042.3652957
GGTACCT3450.042.2309888
GTACATG16050.041.958431
GGTATCA3300.041.3849371
CATGGGT6150.041.2674144
ACATGGG16900.039.7683833
TACATGG17150.039.7367632
ACACCGA608.809595E-539.1658366
ATATACC500.001700200837.5991973
ACCTGTA801.155969E-535.2492527
ATCAACG9550.034.9416663
GACATAT959.274845E-734.7002531
CAACGCA10100.033.969575
TGGGTAC4300.033.8836
AACGCAG9850.033.8774536
TCAACGC9900.033.7063524
GTACCTG4350.033.493549
Produced by FastQC (version 0.11.2)