FastQCFastQC Report
Sun 14 Apr 2019
SRR6576534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203467
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4850.23836789258208949No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2700.13269965154054467No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2310.11353192409579932No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2150.10566824104154482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCTT405.666449E-446.998655
GTCTATA500.001694877437.617412
ATACTTA757.4149375E-637.598926
GTATATG801.1503182E-535.266321
TATACTT550.00271216334.1808365
GTATCAA6200.032.6118661
ACATATA600.004143608331.3478412
TCGTCAG300.00415843731.33243440-41
ACATGGG10950.029.1863753
GTAGAGG650.006128550528.9364681
AACGCAG6900.028.6078726
TACATGG11800.028.2927552
GTACATG11950.027.9376141
ATCAACG7150.027.6075973
TATATGC700.00879935326.869582
GTACTAG700.00879935326.869581
CTAGATT700.00882048426.8563734
CGCGGGG700.00882048426.8563735
CAACGCA7350.026.856375
TCAACGC7350.026.856374
Produced by FastQC (version 0.11.2)