FastQCFastQC Report
Sun 14 Apr 2019
SRR6576534_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576534_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203467
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5300.26048450117218025No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3030.14891849783994457No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2880.14154629497658097No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2710.13319113173143557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2300.11304044390490842No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2200.10812564199599935No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2150.10566824104154482No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2080.10222787970530847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6900.036.2486461
TTACACC550.002713344834.1777954
TATAGAG600.00415540331.3296472
ATTGCCT600.00415540331.3296473
TAGTACT600.00415540331.3296474
ACATGGG12050.030.8096563
GTACATG12750.029.6104371
TTAGAGC650.00614596528.9196743
TACATGG13050.028.448762
CATGGGG5150.028.2879334
AACGCAG8250.027.3422376
CATATAC700.00882431126.8539833
ATCAACG8600.026.2294713
CAACGCA8500.025.9851785
TCAACGC8500.025.9851784
ATGGGAG1659.249561E-825.6333475
TACACTG1101.0151177E-425.6333475
CATGGGC2409.094947E-1225.4553384
ACGCAGA9100.024.271877
CGCAGAG9300.023.7498938
Produced by FastQC (version 0.11.2)