FastQCFastQC Report
Sun 14 Apr 2019
SRR6576535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181735
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4910.270173604424024No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2740.15076897680688914No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2190.12050513109747711No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1950.10729908933337003No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1900.10454783063251437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC405.672054E-446.986523
ATGGGTA2300.040.8578385
GGTACCT2200.038.4435128
TGTAACA500.001700654237.5892142
GGGTACC2400.037.197667
CATGGGT2950.035.040794
ACATGGG12050.034.3138053
TACATGG12350.033.860732
GTACATG12850.033.6401521
TGGGTAC2550.033.1669546
CATGGGC2500.031.950834
GTACCTG2700.031.3243459
GGTCCGA300.004163115331.32434334-35
ATAGTGC600.004157687531.3243436
TAATAGT650.00614934228.9147784
CATAATG650.00614934228.9147782
GTATCAA7150.028.2576261
AACGCAG7750.027.2824946
CATGGGG4200.026.8494364
ACTGCGA3000.026.6256994
Produced by FastQC (version 0.11.2)