FastQCFastQC Report
Sun 14 Apr 2019
SRR6576535_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576535_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181735
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5380.296035436212067No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3070.1689272842325364No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2580.1419649489641511No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2570.14141469722397998No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2340.12875890720004401No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2200.12105538283764823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.11995487935730596No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2150.11830412413679257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG558.663883E-751.3629571
GTGGTCG205.676316E-446.99201690-91
GGGTTAG450.001012546541.7706837
GCTACAC608.8035755E-539.160023
CATGGGT3000.039.1600154
ATGGGTA2000.037.5936135
TATACAG500.001699676337.5936135
TAATGCG250.001701915337.59361318-19
GGTACCT2350.036.0236248
ACATGGG11200.035.2440153
ATTATAC801.1546395E-535.244013
ACCATAT550.002702308634.204258
TACATGG11850.034.1039162
GTACATG11650.033.948051
GTACCTG2500.033.862219
GGGTACC2400.033.286027
CGCCTGT600.00411625631.3884771
GGTTAGC600.00413853331.3538978
TGGGAGT902.5850148E-531.3280126
TGGGTAC2600.030.7255526