Basic Statistics
Measure | Value |
---|---|
Filename | SRR6576535_2.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 181735 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG | 538 | 0.296035436212067 | No Hit |
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA | 307 | 0.1689272842325364 | No Hit |
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA | 258 | 0.1419649489641511 | No Hit |
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA | 257 | 0.14141469722397998 | No Hit |
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT | 234 | 0.12875890720004401 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 220 | 0.12105538283764823 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 218 | 0.11995487935730596 | No Hit |
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG | 215 | 0.11830412413679257 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTACTAG | 55 | 8.663883E-7 | 51.362957 | 1 |
GTGGTCG | 20 | 5.676316E-4 | 46.992016 | 90-91 |
GGGTTAG | 45 | 0.0010125465 | 41.770683 | 7 |
GCTACAC | 60 | 8.8035755E-5 | 39.16002 | 3 |
CATGGGT | 300 | 0.0 | 39.160015 | 4 |
ATGGGTA | 200 | 0.0 | 37.593613 | 5 |
TATACAG | 50 | 0.0016996763 | 37.593613 | 5 |
TAATGCG | 25 | 0.0017019153 | 37.593613 | 18-19 |
GGTACCT | 235 | 0.0 | 36.023624 | 8 |
ACATGGG | 1120 | 0.0 | 35.244015 | 3 |
ATTATAC | 80 | 1.1546395E-5 | 35.24401 | 3 |
ACCATAT | 55 | 0.0027023086 | 34.20425 | 8 |
TACATGG | 1185 | 0.0 | 34.103916 | 2 |
GTACATG | 1165 | 0.0 | 33.94805 | 1 |
GTACCTG | 250 | 0.0 | 33.86221 | 9 |
GGGTACC | 240 | 0.0 | 33.28602 | 7 |
CGCCTGT | 60 | 0.004116256 | 31.388477 | 1 |
GGTTAGC | 60 | 0.004138533 | 31.353897 | 8 |
TGGGAGT | 90 | 2.5850148E-5 | 31.328012 | 6 |
TGGGTAC | 260 | 0.0 | 30.725552 | 6 |