FastQCFastQC Report
Sun 14 Apr 2019
SRR6576537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204265
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3270.16008616258291924No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2090.10231806721660587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG250.005231728356.3950085
GTATCAA6400.041.876171
TACATGG11450.036.547412
GTACATG12100.035.3612941
ATCAACG7400.034.929343
AACGCAG7400.034.929346
CTATAGT550.002712982534.178794
TCAACGC7650.033.7878574
CAACGCA7750.033.3518875
ACATGGG12450.033.2179453
CATGGGT2300.032.6927574
ATGGGGA2600.032.535585
TATCAAC8150.031.730522
ATATACG902.5851394E-531.330566
CCTACGA600.004154849331.3305579
ATAGACT600.004154849331.3305574
CATGGGG5350.029.8665144
GTACTTG953.7271275E-529.6961211
ACGCAGA9050.029.0802977
GGTACCT1303.3115612E-728.9205158
Produced by FastQC (version 0.11.2)