FastQCFastQC Report
Sun 14 Apr 2019
SRR6576537_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576537_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204265
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3880.18994933052652194No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT250.005226067356.410436
GTTATAT502.9585497E-547.112521
GTATCAA5200.046.2065051
TACGCTA954.2382453E-1044.523659
ATGGGTA1750.042.9793745
TATACGC1007.003109E-1042.2974667
CATGGGT2550.038.713044
GGTACCT1600.038.1852158
ATATACG1251.4915713E-1037.6069536
GGGTACC1650.037.0280847
ATACGCT1152.75395E-936.7804078
GTATAGA651.3897289E-436.2403951
CTAAGCT651.4078914E-436.1605344
ATCAACG6550.035.88453
GTACATG11600.035.7405321
TACATGG11600.035.6617662
CAACGCA6750.035.5176775
AACGCAG6900.035.426846
TCAACGC6700.035.0811124
ACATGGG11750.033.6062133
Produced by FastQC (version 0.11.2)