FastQCFastQC Report
Sun 14 Apr 2019
SRR6576538_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576538_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187225
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3670.1960208305514755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.11323274135398584No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2000.10682334089998666No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1880.10041394044598745No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGC200.002154644370.545318
GGTACCT1151.8189894E-1244.9854138
GGGTACC1253.6379788E-1241.353427
TGGGTAC1305.456968E-1239.7629056
CATGGGT2500.035.7143174
GTACCTG1502.7284841E-1134.4888159
GTACATG12650.034.249561
TACATGG12750.033.908332
ATGGGTA1703.6379788E-1233.1711925
GTATCAA6250.032.4000851
ACATGGG13150.032.162183
TTAGCTA600.00415540531.328354
ATAGCCC650.006145972328.9184743
TATAGGA856.800968E-427.642662
ATGGGAC1205.816193E-627.4123065
ATGGGGG1904.4201443E-1027.2061965
ATGGGCC700.00882432526.8528693
ATAGAGT700.00882432526.8528696
CATGGGG5100.026.7212374
ATCAACG7600.026.5878733
Produced by FastQC (version 0.11.2)