FastQCFastQC Report
Sun 14 Apr 2019
SRR6576540_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576540_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254057
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5160.20310402783627296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.12949849836847638No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3030.11926457448525332No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2720.10706258831679503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA5200.048.887131
TACATGG15150.038.463012
GTACATG15500.037.6611981
ACATGGG15700.037.1155783
ATCAACG7000.034.909173
GTATAAG550.002691489234.2374531
TATTACC550.002714995334.1768074
TCAACGC7150.033.519564
CATGGGT3450.032.690864
AACGCAG7550.032.3661166
GGTATCA2650.031.9764881
CAACGCA7650.031.3287435
TACGGGT300.00416180131.32874144-45
CATGGGA7250.031.112684
ATGGGTA2000.030.5455235
CATGGGG7200.030.0233764
GTATTAT1102.9192051E-629.9577731
TATAAGG953.7446494E-529.6798632
GGGTACC1602.1209416E-929.3706937
GTATATA1154.1268904E-628.655261
Produced by FastQC (version 0.11.2)