FastQCFastQC Report
Sun 14 Apr 2019
SRR6576541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265963
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5420.20378774491188623No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3050.1146776055315965No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3020.11354962908374473No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2820.10602978609806628No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2660.1000139117095235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG608.8060035E-539.166519
GTATCAA7600.038.9676971
GTACATG16800.036.6555521
ACATGGG16850.035.982053
TACATGG17450.035.290162
AACGCAG8950.033.0836686
CATGGGT3850.031.7401314
ATCAACG9450.031.3332083
CATGGGG7400.031.1214984
TCAACGC9600.030.8436284
ATGGGTA2600.030.7306445
CAACGCA9900.029.9089725
GGGTATG804.765983E-429.3804051
CATGGGA8200.028.658424
TGGGTAC2300.028.6085826
GTCTTAA1853.237801E-1027.951091
ATGGGGG2600.027.1152745
GTATAAG700.00881611726.8620851
GACGTTT350.00883200326.85703722-23
GTTAGAC700.00882420826.8570373
Produced by FastQC (version 0.11.2)