FastQCFastQC Report
Sun 14 Apr 2019
SRR6576541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265963
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4610.17333238081988847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.12482939356226243No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2940.11054169188947334No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2790.1049018096502145No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2770.10414982535164666No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2720.10226986460522704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA2700.038.2933855
GTATCAA8500.037.1140171
GTACATG17150.036.240311
TACATGG17450.035.2809872
CATGGGG6150.035.15184
ACATGGG17350.034.4008453
GTACTAG550.002689346734.24361
AACGCAG9650.031.1686156
CAACGCA10350.029.5146645
ATAACGC804.7732453E-429.3727663
ATCAACG10500.029.0930273
TCAACGC10500.029.0930274
CATGGGT3850.028.0757884
GTAAGAC856.805247E-427.6449573
ATGGGAG3300.027.0585525
ATGGGTA2450.026.85515
TTAAGGT700.00882730826.85514
AACGCCG700.00882730826.85515
TCAGTCG700.00882730826.85518
TGGGTAC2208.731149E-1125.6344156
Produced by FastQC (version 0.11.2)