FastQCFastQC Report
Sun 14 Apr 2019
SRR6576542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242771
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5010.2063673173484477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.12563279798657995No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2890.11904222497744789No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2710.11162783034217431No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2700.11121591952910356No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2680.11039209790296206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGAC450.001013739641.7663353
GTATCAA8550.039.0426751
CTAGTAC651.4124825E-436.1439443
GTACATG14650.034.6603931
AACGCAG9450.033.810846
ACATGGG15700.033.5194783
TACATGG15300.033.4951862
CATGGGA8600.033.3280754
ATCAACG10350.031.7787343
TCAACGC10400.031.625954
CAACGCA10700.030.739245
CGCAGAG10400.030.7223518
ACGCAGA10500.030.4297587
GTACTTG953.7314458E-529.694431
ATGGGAC2151.8189894E-1228.410825
CTGTGCG1005.3164116E-528.1922769
TATCAAC12350.027.4102442
CATGGGG6200.026.5249924
GCAGAGT12300.025.5946149
CCTAGTA950.001299014124.7453582
Produced by FastQC (version 0.11.2)