FastQCFastQC Report
Sun 14 Apr 2019
SRR6576542_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576542_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242771
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4360.1795931144988487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.12892808449114598No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3040.1252208871735092No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2680.11039209790296206No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2650.1091563654637498No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2560.10544916814611299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC502.9940844E-547.021958
GTACATG17200.035.598541
ACATGGG17250.034.5976683
TACATGG17700.034.2490582
GTATAAG851.72394E-533.2467841
GTATCAA8450.032.3287241
CATGGGG6450.031.3285984
CACCGAC600.00415765431.3285947
CATGGGT3900.030.1236524
CACTCTA1602.1045707E-929.3887169
ATCTAGG650.006081486628.9843751
ATGGGTA2550.027.642885
ATCCGTG350.008837626.8530886-87
CAATACG350.008837626.8530838-39
ATCAACG10550.026.7258193
AACGCAG10400.026.6594316
TCAACGC10900.025.867654
CAACGCA10950.025.3203725
TCTAGGA1501.1577467E-625.0628762
TTGAACA2251.1641532E-1025.0628764
Produced by FastQC (version 0.11.2)