FastQCFastQC Report
Sun 14 Apr 2019
SRR6576543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214830
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6510.30303030303030304No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4160.1936414839640646No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3320.15454079970209003No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3290.1531443466927338No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3130.14569659730950055No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2880.13405948889819858No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC2770.1289391611972257No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2680.12474980216915701No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2610.12149141181399246No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC2560.11916399013173207No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2490.11590559977656752No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2490.11590559977656752No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2480.11544011544011544No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2410.11218172508495089No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2380.11078527207559466No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2360.1098543034026905No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2230.10380300702881348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATG405.667672E-446.9977877
ATAATAG555.270168E-542.725263
GTATCAA4350.042.145761
ATAATGT450.001012347941.775813
GTACATG9900.041.31071
ACATGGG10050.041.152293
TACATGG10050.040.6941222
TGGGTAC1850.038.1063166
GATAATA500.001697397937.6069872
TTATAGT500.001699340937.5982324
CTTATGC500.001699340937.5982323
GTACTTG651.4081277E-436.160561
CATGGGG6050.034.957034
AACGCAG5300.034.583286
ATAGTAT550.00271274134.180216
CAACGCA5500.033.3257065
TCAACGC5500.033.3257064
GTCCTAT1309.098585E-932.5445021
GTACAGG902.5809133E-531.3391531
GGATAAT600.00414974631.3391531
Produced by FastQC (version 0.11.2)