FastQCFastQC Report
Sun 14 Apr 2019
SRR6576543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214830
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7240.3370106595913048No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4830.22482893450635386No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4240.19736535865568122No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4150.19317599962761253No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3480.16198854908532329No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3380.1573337057208025No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3240.1508169250104734No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3150.1466275659824047No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3130.14569659730950055No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2940.13685239491691104No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC2820.13126658287948612No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2750.12800819252432155No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2700.12568077084206117No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2630.12242238048689662No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC2570.11962947446818414No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2550.11869850579527999No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2520.11730205278592376No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2380.11078527207559466No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2360.1098543034026905No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2280.10613042871107387No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC2230.10380300702881348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG250.005159094556.5955121
GGTACCT2600.048.8058788
GGGTACC2650.047.8850177
GTACATG10900.044.5668031
GTATCAA4400.043.9472731
TACATGG11050.043.3830032
TGGGTAC3050.043.1459436
ATGGGTA3050.041.6050155
ACATGGG12050.039.00273
CATGGGT4250.036.4927644
GTACCTG3500.036.2557959
CAACGCA5650.034.9367525
AACGCAG5650.034.9367526
TCAACGC5450.034.4941334
ATCAACG5550.033.872623
TATTATC1554.1836756E-1133.35362
GGTATAG851.7486225E-533.1752367
ATGGGAG2300.032.694445
CCTATAT600.004154277531.332173
CTATTAT2000.030.6559031
Produced by FastQC (version 0.11.2)