FastQCFastQC Report
Sun 14 Apr 2019
SRR6576544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196198
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7130.36340839356160615No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3850.19623033873943668No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3740.19062375763259565No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA3470.17686214946125853No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3260.16615867643910742No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3020.15392613584236334No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2930.1493389331185843No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2870.14628079796939827No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2470.12589323030815808No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2360.12028664920131704No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2320.11824789243519301No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2310.117738203243662No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2290.11671882486060002No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2280.11620913566906901No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2280.11620913566906901No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC2260.115189757286007No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2260.115189757286007No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2250.11468006809447599No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2240.11417037890294499No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2200.11213162213682096No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2120.10805410860457293No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG2120.10805410860457293No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC2040.1039765950723249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAAT156.887214E-494.021941
TATACCG200.0021626770.480514
ATACCGG250.005235315856.38445
TTAGGTA558.790066E-751.2585454
CTTAGGT601.6013346E-646.9870033
TAGGTAT652.7783553E-643.3726165
GTCTTAG802.4401925E-741.1345981
TATAGTA704.624362E-640.2745749
CATGGGG5050.039.0782934
TACATGG10650.037.961912
GTATTAA500.001696641337.6087721
GTACATG10900.037.522521
ACATGGG11050.035.7186243
TATTATC1451.8189894E-1135.6634942
AGGTATA959.418018E-734.6226
GTATCAA4900.034.538671
CTATTAT2200.034.1897931
CCGCAAC550.002715224234.1723639
ATGGGGG1800.033.9350555
TGGGTAC2550.033.1672976
Produced by FastQC (version 0.11.2)