FastQCFastQC Report
Sun 14 Apr 2019
SRR6576545_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576545_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344593
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8940.25943649464730856No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6370.18485575737174No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5120.14858107970852572No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5010.14538890807416285No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4840.1404555519119657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.13813397254152No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4570.1326202215367114No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4550.1320398266941No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4440.12884765505973714No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4390.12739666795320856No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3750.10882403298964285No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3700.10737304588311428No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3500.10156909745699999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10200.054.5277021
GGTATCA3650.045.1970941
ATCAACG12400.043.2072073
TCAACGC12500.042.8615534
CAACGCA12650.042.3533135
AACGCAG12900.041.5325136
GTACATG23350.036.536511
ACGCAGA14900.036.2730947
TACATGG23850.036.0608332
ACATGGG24050.035.1747063
CGCAGAG15600.034.645468
TATCAAC15550.034.4546242
ACCGCGC600.004158137431.3315458
GCAGAGT17750.030.713749
GTAATAG804.6950034E-429.4588221
CATGGGT6250.029.3263244
CATGGGA13000.028.9214254
ATGGGTA4150.028.3116345
CGGTTAT700.00882380426.85950594
CGTCGGA350.0088298226.85950568-69
Produced by FastQC (version 0.11.2)