FastQCFastQC Report
Sun 14 Apr 2019
SRR6576546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310104
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9990.3221499883909914No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6980.22508577767458657No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5760.1857441374506617No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5120.16510589995614375No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4850.156399143513144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.15478678120888476No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4660.15027216675695895No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4480.1444676624616258No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4190.1351159610969223No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3710.11963728297603383No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3600.11609008590666356No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3590.11576761344581173No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3340.10770580192451566No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3330.1073833294636638No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3290.10609343962025644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10550.044.9805151
CCCTATA500.001702849837.587682
GTATATC757.442768E-637.587683
CCTATAC500.001702849837.587683
ATCAACG12550.037.0635533
CAACGCA12850.036.1982545
GTACATG21550.036.1923141
TCAACGC12900.036.057954
AACGCAG13050.035.643496
TACATGG22200.035.3442732
ACATGGG22900.034.058713
ATGGGTA3050.032.3500565
ACGCAGA14450.032.1901447
TATCAAC14950.032.0563852
CGCAGAG14550.031.9689068
GGGTACC3100.031.8282787
GGTACCT3250.031.804968
CATGGGA12200.030.0393374
CCTACAC1102.9668063E-629.8992923
CTTGTAC953.751921E-529.6744883
Produced by FastQC (version 0.11.2)