FastQCFastQC Report
Sun 14 Apr 2019
SRR6576546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310104
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8900.2870004901581405No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5370.17316771147743984No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5060.1631710651910327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.1580115058174032No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4400.141887882774811No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4330.1396305755488481No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4300.1386631581662926No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4280.1380182132445889No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3930.1267316771147744No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3780.12189459020199675No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3750.12092717281944121No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3550.11447772360240434No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3510.11318783375899699No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3320.10706085700281195No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3280.10577096715940458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC352.9373192E-453.7025833
GTATCAA9450.048.7694361
ACCGTTG450.001009878741.802388
GTACATG21450.040.340761
TACATGG21650.039.5017852
ATGGGTA3400.038.697455
ACATGGG21700.038.3280563
TCAACGC12200.037.360714
CAACGCA12450.036.6104975
ATCAACG12500.036.4640583
ATGGGAG4150.036.233075
CATGGGT5100.035.9333464
AACGCAG12500.035.712226
TGGGTAC3500.034.906686
ACGCAGA13600.032.823737
CGCAGAG13900.032.1412168
CATGGGA10550.031.6234424
GGTTATA600.004160774431.3265062
TATCAAC15000.031.0132432
GGGTACC3950.029.7403567
Produced by FastQC (version 0.11.2)