FastQCFastQC Report
Sun 14 Apr 2019
SRR6576548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400141
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5830.14569864122896678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.1244561292144519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9700.047.1452941
ATCAACG12200.035.8263473
TACATGG21650.035.384182
GTACATG21950.035.224731
AACGCAG12050.035.102246
TCAACGC12400.034.8694844
CAACGCA12500.034.2145465
ACATGGG22600.033.064973
TATCAAC13850.032.2368972
ACTATAC953.747319E-529.6829473
ACGCAGA14800.028.262317
CATGGGA12350.028.1607464
CGCAGAG14750.028.0394868
ATGGGTA3800.027.2093685
TACGCCG350.0088362826.85676-77
CATGGGT6000.026.63224
ATGGGGG4000.025.84895
CATGGGG8050.024.5206974
ATACCGT1551.5431342E-624.2570326
GCAGAGT17300.023.9064989
Produced by FastQC (version 0.11.2)