FastQCFastQC Report
Sun 14 Apr 2019
SRR6576549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359779
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6460.17955467106195747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.11618243421656071No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4000.11117936288666098No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3710.10311885907737806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11200.035.246041
GTACATG21850.034.627691
TACATGG22400.033.7774542
ACATGGG22050.033.456443
AACGCAG12750.030.5883876
CATGGGA13000.028.9158084
CAACGCA13500.028.8890325
ATCAACG13200.028.833663
CATGGGT5400.028.7150044
TCAACGC13550.028.4356544
TGGGTAC2500.028.1929116
ATGGGAG5550.027.9389235
GTATTAC909.511165E-426.1081771
GAACTAG909.511165E-426.1081771
CTAATGC909.518887E-426.1045483
ACGCAGA15050.025.9137527
CGCAGAG15300.025.7974368
TATCAAC15350.025.104672
CTAAGGT950.001304754524.7306254
TAATGCA1151.376989E-424.5155744
Produced by FastQC (version 0.11.2)