FastQCFastQC Report
Sun 14 Apr 2019
SRR6576549_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576549_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359779
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5570.15481726281967542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.13035780298461No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC250.00523683256.3899086
GTACATG21100.039.240411
GTATCAA10650.038.4302251
TACATGG21900.037.9795042
ACATGGG22400.036.9219673
CATGGGG6900.032.0087664
ATGGGTA2800.031.8871545
AACGCAG12850.031.0839316
CATGGGT5000.031.014454
TCAACGC13300.030.0322234
ATCAACG13350.029.919743
CATGGGA12900.029.8706234
ATTAGAC953.7497677E-529.67893
CAACGCA13800.028.9440965
TATCAAC15200.027.2056582
ACGCAGA15050.026.2278657
CGCAGAG15200.025.9907118
ATGGGTG2800.025.1740685
GTCTTAA1551.5293535E-624.280721
ATGGGAG5800.023.4957945
Produced by FastQC (version 0.11.2)