FastQCFastQC Report
Sun 14 Apr 2019
SRR6576550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347919
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9050.2601180159749827No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5840.16785516168993359No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4670.1342266447075325No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4510.12962787315438365No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4400.1264662177115938No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4260.12244229260258853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.12186744615844491No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4080.11726867460529605No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3630.10433462961206487No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3570.10261009027963405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG503.00257E-547.006611
GTATCAA9050.043.1110341
CCCGCTA450.001012995141.777653
GTACATG19700.041.5185281
ACATGGG19800.040.3534163
TACATGG20650.039.380842
CATGGGG9200.038.825974
ATGGGAT2300.038.825975
ATGGGCG651.41149E-436.1537365
GGGACCG651.41149E-436.1537365
GACATAT550.002712545734.1866261
TATAGCT550.002714458434.1817134
AACGCAG11150.032.45736
ATCAACG11350.032.299463
TCAACGC11350.032.299464
CATATAG753.2709428E-431.3332373
CAACGCA11800.031.0677035
CATGGGA8800.030.9771774
TAGTACT650.00614844228.922994
ACGCAGA12800.028.2733527