FastQCFastQC Report
Sun 14 Apr 2019
SRR6576550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347919
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8120.23338765632230488No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5090.14629842003454827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.14342418781383023No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4500.12934044993231184No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3950.11353217271836262No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3890.11180763338593178No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3820.10979567083142916No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3660.1051968992782803No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3570.10261009027963405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8850.051.0648841
ATCAACG10650.040.1604843
AACGCAG11300.038.2663046
ACATGGG19950.038.1662333
CAACGCA11350.038.0977335
TCAACGC11300.037.850374
GTACATG20200.037.287481
TACATGG20600.036.7337952
CATGGGG9350.034.6852344
CATGGGA7800.033.7443124
GGTATCA3500.033.6253131
ACGCAGA13200.032.3975527
GTACTAG902.5605405E-531.3836271
TATCAAC13800.031.3340032
ATGGGAT2450.030.6945345
CGCAGAG14050.030.437568
ATGGGTA3150.029.8419085
ACGAGTC404.7823894E-429.37140826-27
GCAGAGT15300.027.950839
CATGGGT5300.027.4911544