FastQCFastQC Report
Sun 14 Apr 2019
SRR6576551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314626
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8070.2564950131266965No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5480.17417505228429947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.14938371272558532No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4550.1446161474258326No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4350.13825939369282894No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4250.13508101682632714No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4060.1290421007799737No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4050.1287242630933235No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3640.11569291794066607No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3440.10933616420766243No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3200.10170805972805808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.043.7453081
GTACGTA450.001014105341.7671136
AACGCAG11700.038.152656
ATGGGTA3600.036.5462235
ATCAACG12000.036.41573
TCAACGC12200.035.8187264
CAACGCA12350.035.383685
GTACATG21350.035.2246861
TACATGG21750.034.360772
ACATGGG21900.034.1145743
ACGCAGA14000.031.8847147
AATAACC753.2689766E-431.3352952
ATGGGAT2700.031.3253355
AGAGTTA902.5925061E-531.3253356
CTAGTAC753.2750785E-431.3253353
GGTACCT3050.030.8118068
ACCCTTG1859.094947E-1230.4787047
CATGGGT5400.030.4551874
GTCCTAC1102.9579733E-629.9109631
CGCAGAG14950.029.8585978