FastQCFastQC Report
Sun 14 Apr 2019
SRR6576551_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576551_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314626
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8610.27365824820580625No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4960.15764749257849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.15669397951853947No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4050.1287242630933235No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4000.1271350746600726No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3950.12554588622682167No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3750.11918913249381806No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3710.11791778174721733No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3510.1115610280142137No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3340.10615778734116063No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3330.10583994965451043No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3210.10202589741470824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCG558.8003617E-751.264325
CATATAG601.6032045E-646.9922943
GTATCAA10300.046.6472631
GTACATG21500.041.8464051
ACATGGG21800.040.3099023
TACATGG22250.040.134632
CATGGGC3600.037.8549044
TGGGTAT500.001701518937.5938346
AATAGTC500.001701518937.5938345
ATCAACG12550.037.069623
TCAACGC12550.037.069624
CAACGCA12600.036.9225165
AACGCAG12600.036.549566
ATGGGTA2900.035.6493265
GGGTGTA702.1531069E-433.6461331
ACGCAGA14500.031.7603077
CCCTTAT600.00411144531.4030591
CATGGGT5450.031.0407814
CATGGGG9100.030.9839294
CATGGGA9700.030.5207654
Produced by FastQC (version 0.11.2)