FastQCFastQC Report
Sun 14 Apr 2019
SRR6576552_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576552_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379865
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8690.2287654824740368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.15347557684967028No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5810.15294907401313626No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4670.12293841233069643No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4600.12109565240282733No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4450.11714688112882207No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4240.11161860134521476No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4170.10977584141734563No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4100.10793308148947653No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3960.1042475616337383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10650.049.5691151
GTACATG25850.039.203121
TACATGG26100.038.360082
ACATGGG25650.037.933543
CATGGGA13300.035.337164
ATCAACG14650.034.972713
CAACGCA14950.034.5807765
TCAACGC15100.034.237264
AACGCAG15350.033.9858326
CAACTAG600.004099573531.4232771
ATGGGAG6300.031.3322815
TATCAAC16950.030.5044652
ACGCAGA17650.029.557087
GGTATCA4350.029.2561551
GTAATAG650.00606352129.0061041
CCCTTAT650.00606352129.0061041
CATGGGG9750.028.9221064
ATGGGTA4250.028.7519765
CGCAGAG18050.028.6416958
ATGGGAT4300.028.4176485