FastQCFastQC Report
Sun 14 Apr 2019
SRR6576554_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576554_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325312
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5720.1758312020460358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.12449586858154632No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3310.1017484753098564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.043.9552271
TCAACGC8750.039.210214
ATCAACG8850.038.767163
GTACATG16800.038.7190171
TACATGG16450.038.570232
CAACGCA9100.038.2185945
AACGCAG9300.037.9020466
ACATGGG17050.036.6616133
CATGGGA9200.034.7380524
CATGGGG5100.034.096984
ATGGGGG2550.033.1754385
ACGCAGA11050.031.899467
TATCAAC11450.031.6060052
CGCAGAG11200.031.4722358
ATGGGAG5750.030.2425375
CTAAGAC650.006148705328.922183
TAAGGTA700.00882821726.8563084
ATGGGAT2700.026.1103025
GCAGAGT13600.025.9183129
AGTAAGG1301.0849666E-525.3069082
Produced by FastQC (version 0.11.2)