FastQCFastQC Report
Sun 14 Apr 2019
SRR6576555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292655
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5770.1971604790623772No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3440.1175445490423878No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3380.11549435341955544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.11481095487861134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6950.043.945651
AGACGTG450.001013984741.7671475
TACATGG17350.038.4570732
GTACATG18050.037.7466241
ACATGGG17800.036.9568863
ATCAACG8800.035.7749823
AACGCAG8900.034.8450626
TCAACGC9150.034.4065444
CAACGCA9250.034.034585
CATGGGG6650.033.209594
ACGTAGC600.004161140431.3253636
ACTATAC902.5918765E-531.3253613
CATGGGA9350.029.6502084
CATGGGT4150.029.4382924
GTCTAGA804.779395E-429.3675251
ACGCAGA10850.029.0156567
CGCAGAG10850.029.0156568
GACGTGT650.006154423628.9157186
GTATACG650.006154423628.9157181
CAGTACG650.006154423628.9157189
Produced by FastQC (version 0.11.2)