FastQCFastQC Report
Sun 14 Apr 2019
SRR6576555_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576555_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292655
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5850.19989407322615368No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3890.1329210162136304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.11583605269002752No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3070.10490167603492166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7800.047.645611
AACGCAG9650.038.9589846
CAACGCA9700.038.273695
ATCAACG9900.037.025793
TCAACGC9900.037.025794
TACATGG15650.033.931972
CGCAGAG11250.033.4410068
ACGCAGA11300.033.2702837
GTACATG15750.033.153761
GTATAGT1001.3965055E-632.929751
ATTATAC1151.1336124E-732.6916733
ACATGGG15900.031.9206393
TATCAAC11800.031.4622692
GAGTGAT600.00414454531.3509439
CATGGGG6800.029.716974
ATGGGGG3100.028.8029445
TATAAGA1154.1863223E-628.6052132
GCAGAGT13600.028.3541629
CTGTGCG1354.5922025E-727.8675069
CATGGGT4000.027.0217084
Produced by FastQC (version 0.11.2)