FastQCFastQC Report
Sun 14 Apr 2019
SRR6576556_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576556_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441936
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10450.23645957785742733No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7460.16880272256616344No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6780.15341587922233083No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6470.14640128887440715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.14278085514644656No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5740.12988305999058686No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5540.12535751783063612No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5230.11834292748271244No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5220.1181166503747149No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5100.11540132507874444No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4880.1104232287027986No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4670.1056714094348503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4510.10205097570688969No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4510.10205097570688969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11800.043.5405241
GTACATG23150.038.482211
TACATGG23200.037.4760132
ACATGGG23500.036.9975973
AACGCAG14700.035.167786
ATCAACG14850.034.1795963
TCAACGC15350.033.372424
CAACGCA15600.032.8376125
CATGGGG11550.032.5524
ATGGGTA4050.032.4917145
GTACACG600.004099999631.423741
CATGGGT6150.031.3312954
ACGCAGA17150.029.8697787
CGCAGAG17950.029.0621788
AGCCGAT650.00615207928.9211987
CATGGGA10500.028.6457564
TATCAAC18550.027.3621032
GTATCGG700.00870701826.9346331
ATGGGAG5600.026.8553965
GCAGAGT19650.026.5478949
Produced by FastQC (version 0.11.2)