FastQCFastQC Report
Sun 14 Apr 2019
SRR6576557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405075
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11950.2950070974510893No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7110.17552305128679874No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6900.1703388261433068No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6330.1562673578966858No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5850.14441770042584706No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5750.141949021786089No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.1345429858668148No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5410.13355551441091157No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5340.13182743936308092No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5100.12590261062766153No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5050.12466827130778252No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5000.12343393198790348No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4510.11133740665308894No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4130.10195642782200827No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4110.10146269209405664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.047.796891
ATCAACG14650.038.167553
AACGCAG15000.037.9034776
TCAACGC15050.037.1531264
CAACGCA15350.036.427015
GTACATG23200.035.65031
TACATGG23750.034.626852
ACTAGGT550.00271840534.172933
ATGGGTA4550.034.079055
ACATGGG24050.033.7999423
CATGGGT6600.033.460994
ACGCAGA17250.032.687157
CGCAGAG17950.031.4124438
ATAGTCG300.004150482431.34840688-89
TGGGTAC4650.031.3251846
GGGTACC4750.030.665717
CTGTTAC1303.326586E-728.9191271
TATCAAC19700.028.3869862
GCAGAGT19750.028.3116239
CATGGGC4400.027.7655054
Produced by FastQC (version 0.11.2)