FastQCFastQC Report
Sun 14 Apr 2019
SRR6576557_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576557_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405075
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10300.25427389989508115No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7170.1770042584706536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.17157316546318582No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6010.14836758624945998No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5830.14392396469789545No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5570.13750540023452448No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5390.13306177868295993No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5200.1283712892674196No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5190.12812442140344382No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5090.12565574276368574No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4910.12121212121212122No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4740.11701536752453248No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4630.11429982102079861No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4320.10664691723754861No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4260.10516571005369377No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4250.10491884218971795No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4170.10294389927791149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.048.56321
AACGCAG16000.037.594676
ATCAACG15800.037.4756973
CAACGCA16050.037.1847575
TCAACGC16000.037.007254
ACGCAGA17550.033.4710357
GTACTAG1309.0713E-932.570031
CGCAGAG18200.032.2955788
GTACATG20800.031.6653081
TACATGG21700.030.534842
ACATGGG21500.030.3817373
GTCCTAG1252.3368557E-730.1091841
GGTATCA5800.030.011851
TATCAAC20150.029.618632
GCAGAGT19950.029.4626279
ATGGGTA4450.028.512815
CATGGGT6100.028.5041544
TGGGTAC4150.028.3092386
TATAAGG1755.5388227E-926.8533342
ACTAGGC1458.6379805E-725.9273573
Produced by FastQC (version 0.11.2)