FastQCFastQC Report
Sun 14 Apr 2019
SRR6576558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293693
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5680.1933992298079968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.12291746824064584No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3130.10657387135546302No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3070.10453092174481517No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3000.10214748053239267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA301.3686348E-462.6743854
GTCGTAC250.00520849856.464641
GTATCAA7600.053.86431
CGCTAAA352.9321184E-453.72095
CCCGCTA405.666334E-447.005793
ATCAACG9050.043.6296843
TCAACGC9250.042.686344
AACGCAG9550.042.3226176
CAACGCA9450.042.2803425
TATCAAC10450.038.2474062
ACGCAGA10850.037.2517057
CGCAGAG11050.037.002788
TACATGG14550.034.9027822
ACATGGG14600.034.449453
GTACATG14850.033.9041371
GCAGAGT12400.032.595249
GGTATCA3600.031.3692471
TTAGACT753.2672888E-431.3371944
ACTGATC753.2705543E-431.331868
CTATACA1303.3137803E-728.926644
Produced by FastQC (version 0.11.2)