FastQCFastQC Report
Sun 14 Apr 2019
SRR6576558_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576558_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293693
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5340.18182251534765895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.17058629248909574No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3260.11100026217853336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6700.049.9429551
ACCGTCC652.7807218E-643.3780148
AACGCAG9200.036.266226
ATCAACG9100.036.1483463
CAACGCA9250.036.0701875
GTACATG12250.035.3950421
TCAACGC9550.034.937094
TACATGG12500.034.5926252
ACGCAGA10700.032.060547
ACATGGG12950.031.9333633
TATCAAC10600.031.925112
GTATAAG1052.01478E-631.4195141
CGCGGCA300.00416243931.32856640-41
GCACCGT753.2725703E-431.3285666
CGCAGAG10900.030.610028
TGCCGTA505.3246797E-528.19571114-15
CATGGGT3700.027.9416924
TAGGCCA856.809978E-427.6428534
CATGGGG6300.027.5989744
AGTAGGC700.00882472526.857632
Produced by FastQC (version 0.11.2)