FastQCFastQC Report
Sun 14 Apr 2019
SRR6576559_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576559_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377859
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6650.17599157357638698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.10930002990533506No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3920.10374240126608074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3910.1034777522832591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAC250.00523417456.3976973
CTACTAG352.8936035E-453.8689161
TACGATA352.9355753E-453.712092
GTATCAA7700.041.625981
TACATGG18900.040.0354042
GTACATG19350.039.9493031
ACATGGG19300.038.962143
ATCAACG9450.032.3267253
CATGGGG8600.031.149894
CATGGGA9750.030.8500234
CATGGGT5300.030.149714
TCAACGC10150.030.0972964
GTATAGA1102.8958639E-629.9951921
AACGCAG10700.029.8679376
ACCGTCT804.7760218E-429.3738028
CAACGCA10300.029.202695
GTCCTAC650.006150237828.92189694
GCACCGT650.006150237828.9218966
GGTATCA4300.028.5004141
GATTCGC505.3226344E-528.19884952-53
Produced by FastQC (version 0.11.2)